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Protocols

Morrissey Lab protocols (pdf files):

  • Clotting Assay - measure tissue factor procoagulant activity in cell lysates and other samples using a clotting assay
  • Factor VIIa enzymatic activity - I - measure the enzymatic activity of coagulation factor VIIa using a chromogenic substrate (amidolytic activity)
  • Factor VIIa enzymatic activity - II - measure the enzymatic activity of coagulation factor VIIa using a fluorgenic substrate (amidolytic activity)
  • Relipidation - I - rapidly reconstitute tissue factor into liposomes using Bio-Beads SM-2
  • Relipidation - II- reconstitute tissue factor into liposomes using detergent dialysis
  • SUV - prepare small, unilamellar phospholipid vesicles by sonication
  • Membrane Extrusion - prepare large, unilamellar phospholipid vesicles by extrusion

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