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Information on Tissue Factor DNA Clones and Probes Human Tissue Factor cDNA clones Isolation and sequencing of cDNA clones for human tissue factor was reported almost simultaneously by four groups (Fisher et al., 1987; Morrissey et al., 1987; Scarpati et al., 1987; Spicer et al., 1987). The TF mRNA sequence contains: (1) a typically short 5' noncoding sequence; (2) a coding sequence that encompasses the leader peptide, extracellular domain, transmembrane domain, and cytoplasmic domains of tissue factor; and (3) a long 3' noncoding sequence. Note that the 3' noncoding sequence contains an Alu-family repeat that should be removed by subcloning before using the tissue factor cDNA as a hybridization probe. A human tissue factor cDNA clone is available for a nominal fee from the American Type Culture Collection (ATCC). The clone, designated pHTF8, was deposited by Dr. Evan Sadler. The locus for human tissue factor is identified in the ATCC catalog as "F3", because tissue factor is sometimes known as coagulation factor III. The ATCC numbers for this clone are 61350 (bacteria) or 61351 (DNA).
Isolation of mouse tissue factor cDNA clones was reported by Hartzell et al. (1989) and Ranganathan et al. (1991). A mouse tissue factor cDNA clone is available for a nominal fee from the ATCC. The clone, designated pGEM482/mtf, was deposited by Dr. Daniel Nathans. The locus is identified in the ATCC catalog as "Cf3". The ATCC catalog number for this clone is 63028.
The sequence of the entire tissue factor gene was reported by Mackman et al. (1989). The gene is about 13 kbp in length, spanning six exons and five introns. The 5' end of the tissue factor gene is contained within a highly GC-rich region about 1 kbp long called a CpG island (sometimes also called an HTF island).
American Type Culture Collection
Fisher, K.L., Gorman, C.M., Vehar, G.A., O'Brien, D.P., and Lawn, R.M. (1987). Cloning and expression of human tissue factor. Thromb. Res. 48:89-99. Hartzell, S., Ryder, K., Lanahan, A., Lau, L.F., and Nathans, D. (1989). A growth factor-responsive gene of murine BALB/c 3T3 cells encodes a protein homologous to tissue factor. Molec. Cell. Biol. 9:2567-2573. Mackman, N., Morrissey, J.H., Fowler, B., and Edgington, T.S. (1989). Complete sequence of the human tissue factor gene, a highly regulated cellular receptor that initiates the coagulation protease cascade. Biochemistry 28:1755-1762. Morrissey, J.H., Fakhrai, H., and Edgington, T.S. (1987). Molecular cloning of the cDNA for tissue factor, the cellular receptor for the initiation of the coagulation protease cascade. Cell 50:129-135. Ranganathan, G., Blatti, S.P., Subramaniam, M., Fass, D.N., Maihle, N.J., and Getz, M.J. (1991). Cloning of murine tissue factor and regulation of gene expression by transforming growth factor type beta1. J. Biol. Chem. 266:496-501. Scarpati, E.M., Wen, D., Broze, G.J., Jr., Miletich, J.P., Flandermeyer, R.R., Siegel, N.R., and Sadler, J.E. (1987). Human tissue factor: cDNA sequence and chromosome localization of the gene. Biochemistry 26:5234-5238. Spicer, E.K., Horton, R., Bloem, L., Bach, R., Williams, K.R., Guha, A., Kraus, J., Lin, T.-C., Nemerson, Y., and Konigsberg, W.H. (1987). Isolation of cDNA clones coding for human tissue factor: Primary structure of the protein and cDNA. Proc. Natl. Acad. Sci. USA 84:5148-5152. |
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