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Protocols
Morrissey Lab protocols (pdf files):
- Clotting Assay - measure tissue factor procoagulant activity in cell lysates and other samples using a clotting assay
- Factor VIIa enzymatic activity - I - measure the enzymatic activity of coagulation factor VIIa using a chromogenic substrate (amidolytic activity)
- Factor VIIa enzymatic activity - II - measure the enzymatic activity of coagulation factor VIIa using a fluorgenic substrate (amidolytic activity)
- Relipidation - I - rapidly reconstitute tissue factor into liposomes using Bio-Beads SM-2
- Relipidation - II- reconstitute
tissue factor into liposomes using detergent dialysis
- SUV - prepare small, unilamellar phospholipid
vesicles by sonication
- Membrane Extrusion - prepare large,
unilamellar phospholipid vesicles by extrusion
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